Arenibacter gen. nov., new genus of the family flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov

Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):1987-1995. doi: 10.1099/00207713-51-6-1987.

Abstract

Five dark-orange-pigmented, Gram-negative, rod-shaped, non-motile, aerobic bacterial strains were isolated from sandy sediment samples collected in the South China Sea in the Indian Ocean, from a holothurian, Apostichopus japonicus, in the Sea of Japan and from a brown alga, Chorda filum, from the Sea of Okhotsk in the Pacific Ocean. Phenotypic data were collected, demonstrating that the bacteria are chemo-organotrophic and require seawater-based media for growth. Polar lipids were analysed and 27% of the total extract comprised phosphatidylethanolamine as the major component. The predominant cellular fatty acids were branched-chain saturated and unsaturated [i-C15:0, i-C15:1, a-C15:0, C15:0, C16:1(n-7)]. The DNA base composition was 37.5-38.2 mol % G+C. The level of DNA homology of the five isolates was 83-94%, indicating that these isolates belong to the same species. A 16S rDNA sequence of the type strain KMM 426T was determined and phylogenetic analysis, based on neighbour-joining and Fitch-Margoliash methods, revealed that the type strain formed a distinct phyletic line in a clade corresponding to the family Flavobacteriaceae and represented a new genus. From the results of this polyphasic taxonomic analysis, it is proposed that the bacterial strains be classified in a new genus, Arenibacter gen. nov., and species, Arenibacter latericius sp. nov. The type strain is KMM 426T (VKM B 2137DT = LMG 19694T = CIP 106861T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Geologic Sediments / microbiology
  • Gram-Negative Aerobic Rods and Cocci / classification*
  • Gram-Negative Aerobic Rods and Cocci / genetics*
  • Gram-Negative Aerobic Rods and Cocci / isolation & purification
  • Molecular Sequence Data
  • Phaeophyta / microbiology
  • Phenotype
  • Phylogeny
  • Pigments, Biological / metabolism
  • RNA, Ribosomal, 16S
  • Sea Cucumbers / microbiology
  • Seawater / microbiology

Substances

  • DNA, Ribosomal
  • Fatty Acids
  • Pigments, Biological
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AF052740