Evaluating the oncogenic risk of residual DNA in vaccines: the potential role of transgenic mice

Dev Biol (Basel). 2001:106:135-42; discussion 143-60.

Abstract

There is a general consensus that residual DNA from immortalised cells is very unlikely to pose a safety risk for biotechnology products but there is little experimental data to support this contention. Transgenic mice primed with one or more oncogenes or tumour suppressor deletions offer a potentially sensitive and quantitative method for determining the presence of oncogenic DNA. Separate lines of mice transgenic for myc and Cbfa1 may provide a means of detecting oncogenic DNA containing genes belonging to different complementation groups.

MeSH terms

  • Animals
  • Cell Line, Transformed
  • Core Binding Factor Alpha 1 Subunit
  • DNA / physiology*
  • Genes, Tumor Suppressor*
  • Genes, myc
  • Genetic Complementation Test
  • Mice
  • Mice, Transgenic
  • Neoplasm Proteins*
  • Neoplasms, Experimental / genetics*
  • Neoplasms, Experimental / pathology
  • Transcription Factors / genetics

Substances

  • Core Binding Factor Alpha 1 Subunit
  • Neoplasm Proteins
  • Transcription Factors
  • DNA