Overexpression of Pti5 in tomato potentiates pathogen-induced defense gene expression and enhances disease resistance to Pseudomonas syringae pv. tomato

Mol Plant Microbe Interact. 2001 Dec;14(12):1453-7. doi: 10.1094/MPMI.2001.14.12.1453.

Abstract

The tomato Pti5 gene encodes a pathogen-inducible ethylene response element-binding protein-like transcription factor that interacts with the disease resistance gene product Pto. Overexpression of Pti5 or Pti5-VP16, a translational fusion with a constitutive transcriptional activation domain, in tomato enhanced resistance to Pseudomonas syringae pv. tomato. Constitutive expression of Pti5 or Pti5-VP16 did not affect the basal level of pathogenesis-related gene expression, but it accelerated pathogen-induced expression of GluB and Catalase. The results demonstrate a positive role of Pti5 in defense gene regulation and disease resistance and suggest that a pathogen-activated posttranscriptional regulatory step is necessary for the pathogen induction of the defense gene expression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation, Plant
  • Immunity, Innate / genetics
  • Lycopersicon esculentum / genetics*
  • Lycopersicon esculentum / microbiology*
  • Plant Diseases / genetics
  • Plant Diseases / microbiology
  • Plant Leaves / microbiology
  • Plant Proteins / genetics*
  • Pseudomonas / pathogenicity*
  • Transcription Factors / genetics*

Substances

  • DNA-Binding Proteins
  • Plant Proteins
  • Pti5 protein, Lycopersicon esculentum
  • Transcription Factors