Knowledge of dispersion rates and patterns of radioactive aerosols and gases through workrooms is critical for understanding human exposure and for developing strategies for worker protection. The dispersion within rooms can be influenced by complex interactions between numerous variables, but especially ventilation design and room furnishings. For this study, dependence of airflow and aerosol dispersion on workroom geometry (furnishings) and ventilation rate were studied in an experimental room that was designed to approximate a plutonium laboratory. Three different configurations of simulated gloveboxes and two ventilation rates (approximately 6 and 12 air exchanges per hour) were studied. A sonic anemometer was used to measure airflow parameters including all three components of air velocity vectors and turbulence intensity distributions at multiple locations and heights. Aerosol dispersion rates and patterns were measured by releasing aerosols multiple times from six different locations. Aerosol particle concentrations resolved in time and space were measured using 16 multiplexed laser particle counters. Comparisons were made of air velocities, turbulence, and aerosol transport across different ventilation rates and room configurations. A strong influence of ventilation rate on aerosol dispersion rates and air velocity was found, and changes in room geometry had significant effects on aerosol dispersion rates and patterns. These results are important with regards to constant evaluation of placement of air sampling equipment, benchmarking numerical models of room airflow, and design of ventilation and room layouts with consideration of worker safety.