Site-directed mutagenesis facilitated by DpnI selection on hemimethylated DNA

Methods Mol Biol. 2002;182:19-27. doi: 10.1385/1-59259-194-9:019.
No abstract available

MeSH terms

  • DNA Methylation*
  • DNA Primers
  • DNA, Single-Stranded / genetics
  • Deoxyribonucleases, Type II Site-Specific
  • Indicators and Reagents
  • Molecular Biology / methods
  • Mutagenesis, Site-Directed*
  • Plasmids
  • Templates, Genetic

Substances

  • DNA Primers
  • DNA, Single-Stranded
  • Indicators and Reagents
  • endodeoxyribonuclease DpnI
  • Deoxyribonucleases, Type II Site-Specific