Comparative Genomic Hybridization Detects an Increased Number of Chromosomal Alterations in Large cell/anaplastic Medulloblastomas

Brain Pathol. 2002 Jan;12(1):36-44. doi: 10.1111/j.1750-3639.2002.tb00420.x.

Abstract

We correlate chromosomal changes in medulloblastomas with histologic subtype, reporting the analysis of 33 medulloblastoma specimens by comparative genomic hybridization, and a subset by fluorescence in situ hybridization. Of the 33 tumors, 5 were desmoplastic/nodular, 10 were histologically classic, and 18 were large cell/anaplastic. Chromosomal gains and losses were more common in anaplastic medulloblastomas than in non-anaplastic ones. We identified 4 medulloblastomas with c-myc amplification and 5 medulloblastomas with N-myc amplification; all 9 were of the large cell/anaplastic subtype. Additional regions with high level gains included 2q14-22, 3p23, 5p14-pter, 8q24, 9p22-23, 10p12-pter, 12q24, 12p11-12, 17p11-12, and Xp11. The majority of these high level gains occurred in anaplastic cases. We also found loss of chromosome 17p in 7 large cell/anaplastic cases but no nonanaplastic medulloblastomas. Finally, we detected a significantly increased overall number of chromosomal alterations in large cell/anaplastic medulloblastomas (6.8/case) compared to non-anaplastic ones (3.3/case). These findings support an association between myc oncogene amplification, 17p loss, and large cell/anaplastic histology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Anaplasia / genetics*
  • Anaplasia / pathology
  • Cerebellar Neoplasms / genetics*
  • Cerebellar Neoplasms / pathology
  • Child
  • Child, Preschool
  • Chromosome Aberrations / classification*
  • Chromosomes, Human, Pair 17 / genetics*
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Medulloblastoma / genetics*
  • Medulloblastoma / pathology
  • Nucleic Acid Hybridization