Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD

Quan Du; Nicolas Carrasco; Marianna Teplova; Christopher J Wilds; Martin Egli; Zhen Huang

doi:10.1021/ja0171097

Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD

J Am Chem Soc. 2002 Jan 9;124(1):24-5. doi: 10.1021/ja0171097.

Authors

Quan Du¹, Nicolas Carrasco, Marianna Teplova, Christopher J Wilds, Martin Egli, Zhen Huang

Affiliation

¹ Department of Chemistry, Brooklyn College, The City University of New York, 2900 Bedford Avenue, Brooklyn, New York 11210, USA.

PMID: 11772055
DOI: 10.1021/ja0171097

Abstract

We have developed a route for the synthesis of 2'-selenium uridine analogues and oligonucleotides containing selenium labels, and have demonstrated for the first time a new strategy to covalently derivatize nucleotides with selenium for phase and structure determination in X-ray crystallography.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Crystallography, X-Ray
DNA / chemistry
Oligonucleotides / chemistry*
Organoselenium Compounds / chemical synthesis
Organoselenium Compounds / chemistry*
RNA / chemistry
Spectrometry, Fluorescence

Substances

Oligonucleotides
Organoselenium Compounds
RNA
DNA

Grants and funding

GM-55237/GM/NIGMS NIH HHS/United States