Objective: To study the effect of recombinant human interleukin-1(rhIL-1) and dexamethasone on the amount of 6-keto-prostaglandin F1 alpha produced by condylar chondrocytes.
Methods: The concentration of 6-keto-prostaglandin F1 alpha of the chondrocytes supernate was detected respectively at 4, 8, 12, 24, and 72 hours, using the method of radioimmunoassay, after being stimulated by rhIL-1 and dexamethasone for four hours.
Results: In normal control group, the concentration of 6-keto-prostaglandin F1 alpha was 1,516.49 ng/L, 1,513.22 ng/L, 1,506.76 ng/L, 1,526.79 ng/L and 2,114.36 ng/L at 4, 8, 12, 24 and 72 hours respectively. In rhIL-1 group, the concentration of 6-keto-prostaglandin F1 alpha was 1,664.32 ng/L at four hours, which was sharply higher than that of the control group(P < 0.01); the concentration of 6-keto-prostaglandin F1 alpha was 1,146.11 ng/L, 949.24 ng/L, 1,392.33 ng/L and 1,481.98 ng/L at 8, 12, 24 and 72 hours respectively, which were sharply lower than that of the control group(P < 0.01). After the condylar chondrocytes were stimulated with dexamethasone for 4 hours, the concentration of 6-keto-prostaglandin F1 alpha decreased more markedly than that of the control group(P < 0.01) throughout the observation period, both in the presence and absence of rhIL-1.
Conclusion: rhIL-1 could enhance the production of 6-keto-prostaglandin F1 alpha synthesized by condylar chondrocytes. Dexamethasone could inhibit the activity of rhL-1 and the production of 6-keto-prostaglandin F1 alpha synthesized by condylar chondrocytes.