Two Novel RNA Binding Proteins From Trypanosoma Brucei Are Associated With 5S rRNA

Biochem Biophys Res Commun. 2002 Jan 11;290(1):569-76. doi: 10.1006/bbrc.2001.6226.

Abstract

We have previously reported the identification of two closely related RNA binding proteins from Trypanosoma brucei which we have termed p34 and p37. The predicted primary structures of the two proteins are highly homologous with one major difference, an 18-amino-acid insert in the N-terminal region of p37. These two proteins have been localized to the nucleus based on immunofluorescence microscopy. To gain insight into their function, we have utilized UV crosslinking, coimmunoprecipitation, and sucrose density gradients to identify T. brucei RNA species that associate with p34 and p37. These experiments have demonstrated a specific interaction of both p34 and p37 with the 5S ribosomal RNA and indicate that other RNA species are unlikely to be specifically bound. This suggests a role for p34 and p37 in the import and/or assembly pathway of T. brucei 5S rRNA in ribosome biogenesis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Centrifugation, Density Gradient
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Nuclear Proteins / metabolism
  • Nuclear Proteins / physiology*
  • Poly A
  • Precipitin Tests
  • Protein Binding
  • Protein Structure, Tertiary
  • Protozoan Proteins*
  • RNA / metabolism*
  • RNA, Ribosomal, 5S / metabolism*
  • RNA-Binding Proteins / chemistry*
  • RNA-Binding Proteins / metabolism
  • RNA-Binding Proteins / physiology*
  • Sucrose / pharmacology
  • Trypanosoma brucei brucei / metabolism*
  • Ultraviolet Rays

Substances

  • Nuclear Proteins
  • Protozoan Proteins
  • RNA, Ribosomal, 5S
  • RNA-Binding Proteins
  • p34 protein, Trypanosoma
  • p37 protein, Trypanosoma
  • Poly A
  • Sucrose
  • RNA