Meiotic silencing by unpaired DNA

Cell. 2001 Dec 28;107(7):905-16. doi: 10.1016/s0092-8674(01)00609-2.

Abstract

The silencing of gene expression by segments of DNA present in excess of the normal number is called cosuppression in plants and quelling in fungi. We describe a related process, meiotic silencing by unpaired DNA (MSUD). DNA unpaired in meiosis causes silencing of all DNA homologous to it, including genes that are themselves paired. A semidominant Neurospora mutant, Sad-1, fails to perform MSUD. Sad-1 suppresses the sexual phenotypes of many ascus-dominant mutants. MSUD may provide insights into the function of genes necessary for meiosis, including genes for which ablation in vegetative life would be lethal. It may also contribute to reproductive isolation of species within the genus Neurospora. The wild-type allele, sad-1(+), encodes a putative RNA-directed RNA polymerase.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA, Fungal / genetics*
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal
  • Gene Silencing*
  • Meiosis / genetics*
  • Mutation
  • Neurospora / genetics*
  • RNA Replicase / genetics
  • Schizosaccharomyces pombe Proteins*

Substances

  • DNA, Fungal
  • Fungal Proteins
  • SAD1 protein, S pombe
  • Schizosaccharomyces pombe Proteins
  • RNA Replicase