A glutathione S-transferase cDNA identified by mRNA differential display is upregulated during somatic embryogenesis in Cichorium

Biochim Biophys Acta. 2001 Dec 30;1522(3):212-6. doi: 10.1016/s0167-4781(01)00314-1.

Abstract

CHI-GST1, a cDNA encoding a glutathione S-transferase, was isolated by differential display in leaf tissues of chicory, during the early stages of somatic embryogenesis. Expression analysis of the gene by Northern blot indicated that the transcript accumulation is specific of the leaf developing somatic embryogenesis and is not observed in leaf tissue of the non-embryogenic cultivar.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chicory / embryology*
  • Chicory / enzymology
  • Chicory / genetics
  • DNA, Complementary / analysis
  • DNA, Complementary / isolation & purification
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression Profiling
  • Genotype
  • Glutathione Transferase / biosynthesis
  • Glutathione Transferase / genetics*
  • Molecular Sequence Data
  • Plant Leaves / metabolism
  • Sequence Alignment
  • Up-Regulation

Substances

  • DNA, Complementary
  • Glutathione Transferase

Associated data

  • GENBANK/AJ296343