BMP-12 gene transfer augmentation of lacerated tendon repair

J Orthop Res. 2001 Nov;19(6):1199-202. doi: 10.1016/S0736-0266(01)00042-0.


Bone morphogenetic protein (BMP) 12 is a recently discovered member of the human BMP family. It is the human homolog of mouse growth/differentiation factor (GDF)-7. Previously we reported that injection of mesenchymal progenitor cells transferred with the BMP-12 gene into the muscles of nude mice induced tendon-like tissue formation. In this study, we further investigated the effect of BMP-12 gene transfer on tendon cells. We observed that adenovirus mediated in vitro BMP-12 gene transfer into chicken tendon cells increased type I collagen synthesis. No change in alkaline phosphatase activity was observed following BMP-12 gene transfer. We also determined that BMP-12 gene transfer into a complete tendon laceration chicken model resulted in a two-fold increase of tensile strength and stiffness of repaired tendons, indicating improved tendon healing in vivo. We conclude that BMP-12 gene transfer is a promising procedure for improving the tendon repair process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Biomechanical Phenomena
  • Bone Morphogenetic Proteins / genetics*
  • Cells, Cultured
  • Chickens
  • Collagen / biosynthesis
  • Gene Transfer, Horizontal
  • Genetic Therapy*
  • Growth Differentiation Factors
  • Tendon Injuries / physiopathology
  • Tendon Injuries / therapy*
  • Tendons / physiology*
  • Tensile Strength
  • Transforming Growth Factor beta*
  • Wound Healing


  • Bone Morphogenetic Proteins
  • GDF7 protein, human
  • Growth Differentiation Factors
  • Transforming Growth Factor beta
  • growth differentiation factor 7
  • Collagen
  • Alkaline Phosphatase