Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly

Nat Cell Biol. 2001 Dec;3(12):1086-91. doi: 10.1038/ncb1201-1086.

Abstract

Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97-Ufd1-Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Animals
  • Carrier Proteins / metabolism
  • Endoplasmic Reticulum / metabolism
  • Golgi Apparatus / metabolism
  • Membrane Fusion / physiology*
  • Nuclear Envelope / enzymology*
  • Nuclear Pore Complex Proteins*
  • Nuclear Proteins / metabolism*
  • Nucleocytoplasmic Transport Proteins
  • Oocytes / metabolism
  • Saccharomyces cerevisiae Proteins / metabolism
  • Vesicular Transport Proteins
  • Xenopus

Substances

  • Carrier Proteins
  • NPL4 protein, S cerevisiae
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • Nucleocytoplasmic Transport Proteins
  • Saccharomyces cerevisiae Proteins
  • UFD1 protein, S cerevisiae
  • Vesicular Transport Proteins
  • Adenosine Triphosphatases
  • p97 ATPase