Embryonic expression of an Nkx2-5/Cre gene using ROSA26 reporter mice

Genesis. 2001 Dec;31(4):176-80. doi: 10.1002/gene.10022.


Nkx2-5, one of the earliest cardiac-specific markers in vertebrate embryos, was used as a genetic locus to knock in the Cre recombinase gene by homologous recombination. Offspring resulting from heterozygous Nkx2-5/Cre mice mated to ROSA26 (R26R) reporter mice provided a model system for following Nkx2-5 gene activity by beta-galactosidase (beta-gal) activity. beta-gal activity was initially observed in the early cardiac crescent, cardiomyocytes of the looping heart tube, and in the epithelium of the first pharyngeal arch. In later stage embryos (10.5-13.5 days postcoitum, dpc), beta-gal activity was observed in the stomach and spleen, the dorsum of the tongue, and in the condensing primordium of the tooth. The Nkx2-5/Cre mouse model should provide a useful genetic resource to elucidate the role of loxP manipulated genetic targets in cardiogenesis and other developmental processes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Embryo, Mammalian / metabolism
  • Female
  • Gene Expression
  • Genes, Reporter
  • Genetic Vectors
  • Heart / embryology
  • Homeobox Protein Nkx-2.5
  • Homeodomain Proteins / genetics*
  • Integrases / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Models, Genetic
  • Myocardium / metabolism
  • Proteins / genetics
  • RNA, Untranslated
  • Recombinant Fusion Proteins / genetics
  • Transcription Factors*
  • Viral Proteins / genetics
  • Xenopus Proteins*
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism


  • Gt(ROSA)26Sor non-coding RNA, mouse
  • Homeobox Protein Nkx-2.5
  • Homeodomain Proteins
  • NKX2-5 protein, human
  • Nkx2-5 protein, mouse
  • Proteins
  • RNA, Untranslated
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Viral Proteins
  • Xenopus Proteins
  • Cre recombinase
  • Integrases
  • beta-Galactosidase