Replication-dependent recruitment of the beta-subunit of DNA polymerase III from cytosolic spaces to replication forks in Escherichia coli

J Bacteriol. 2002 Feb;184(3):867-70. doi: 10.1128/JB.184.3.867-870.2002.


The beta-subunit of DNA polymerase III is located as one or two condensed clusters within the nucleoid-occupied space in exponentially growing cells of Escherichia coli. When chromosome replication is terminated after incubation at nonpermissive temperature in a temperature-sensitive dnaC mutant, the beta-subunit is located in the cytosolic spaces of the cell poles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins
  • Cell Compartmentation
  • Chromosomes, Bacterial
  • Cytosol / enzymology
  • DNA Polymerase III / isolation & purification*
  • DNA Replication*
  • DNA, Bacterial / biosynthesis*
  • DNA-Binding Proteins
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli Proteins
  • Protein Subunits
  • Transcription Factors / isolation & purification


  • Bacterial Outer Membrane Proteins
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Protein Subunits
  • SeqA protein, E coli
  • Transcription Factors
  • beta subunit, DNA polymerase III
  • DNA Polymerase III