The role of DSC1 components cdc10+, rep1+ and rep2+ in MCB gene transcription at the mitotic G1-S boundary in fission yeast

Curr Genet. 2001 Dec;40(4):251-9. doi: 10.1007/s002940100248.


In this paper, we describe the transcription profile of a group of genes at the G1-S boundary of fission yeast in synchronously dividing mitotic cells, under a variety of different conditions. This transcription profile is unaffected in cells where either cdc10+ or cdc10-C4 are constitutively overexpressed. In contrast, overexpression of either rep1+ or rep2+ results in constitutive expression of MCB-regulated genes, suggesting that these polypeptides have important regulatory properties in controlling MCB transcription. Finally, we examine the pattern of MCB-regulated transcription in cells where the G1 period is extended. Surprisingly, we find that the wee1-50 mutation causes MCB transcription throughout the cell cycle, whereas cells re-fed after nitrogen starvation have normal expression patterns. The implications of these observations for understanding MCB-regulated transcription are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • G1 Phase
  • Gene Expression Regulation, Fungal
  • Membrane Glycoproteins / chemistry*
  • S Phase
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors*
  • Transcription, Genetic / genetics*


  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • DSC1 protein, S pombe
  • Fungal Proteins
  • Membrane Glycoproteins
  • Rep2 protein, S pombe
  • Schizosaccharomyces pombe Proteins
  • Trans-Activators
  • Transcription Factors
  • cdc10 protein, S pombe
  • rep1 protein, S pombe