Renal handling of human beta2-microglobulin in the rat: the importance of sham-operation

Acta Physiol Scand. 1975 Aug;94(4):467-71. doi: 10.1111/j.1748-1716.1975.tb05906.x.


The plasma disappearance rates of human beta2-microglobulin and (hydroxy-methyl-C14)-inulin was measured in unoperated, sham-operated, ureter-ligated and nephrectomised rats. Judging from the C14-activity curves in ureter-ligated and nephrectomised rats a substantial glomerular filtration continued after obstruction of the urine flow. Almost 90% of the injected protein had disappeared from plasma 120 min after injection into nephrectomised rats indicating a considerable extra-renal elimination and invalidating attempts to evaluate different renal mechanisms of elimination before and after ureter-ligation. Sham-operation induced an increase of the plasma disappearance of both beta2-microglobulin and inulin. The latter phenomenon may explain earlier findings of similarity between the disappearance curves of low molecular weight proteins in unoperated and ureter-ligated animals and stresses the importance of using sham-operated animals as controls.

MeSH terms

  • Animals
  • Glomerular Filtration Rate
  • Humans
  • Injections, Intravenous
  • Inulin / administration & dosage
  • Inulin / metabolism
  • Kidney / metabolism*
  • Kidney / physiology
  • Ligation
  • Male
  • Nephrectomy
  • Rats
  • Serum Globulins / administration & dosage
  • Serum Globulins / metabolism*
  • Surgical Procedures, Operative
  • Time Factors
  • Ureter / physiology


  • Serum Globulins
  • Inulin