Cloning and Expression of the Cdx Family From the Frog Xenopus Tropicalis

Dev Dyn. 2002 Jan;223(1):134-40. doi: 10.1002/dvdy.1234.


The caudal-related (Cdx) homeodomain transcription factors have a conserved role in the development of posterior structures in both vertebrates and invertebrates. A particularly interesting finding is that Cdx proteins have an important function in the regulation of expression from a subset of Hox genes. In this study, we report the cloning of cDNAs from the Cdx genes of the amphibian Xenopus tropicalis. Xenopus tropicalis is a diploid species, related to the commonly used laboratory animal Xenopus laevis, and has attracted attention recently as a potential genetic model for animal development. The Xenopus tropicalis cDNAs, Xtcad1, Xtcad2, and Xtcad3, show between 88 and 94% sequence identity with their Xenopus laevis orthologues. This finding corresponds to between 90 and 95% identity at the level of derived amino acid sequence. We also present a detailed description of Xtcad1, Xtcad2, and Xtcad3 expression during normal development. In common with the Cdx genes of other vertebrates, the Xenopus tropicalis Cdx genes show overlapping and dynamic patterns of expression in posterior regions of the embryo through the early stages of development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CDX2 Transcription Factor
  • Cloning, Molecular
  • Embryo, Nonmammalian / anatomy & histology
  • Embryo, Nonmammalian / physiology
  • Female
  • Fetal Proteins
  • Homeodomain Proteins / chemistry
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Sequence Alignment
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*
  • Xenopus / genetics*
  • Xenopus / metabolism
  • Xenopus Proteins / chemistry
  • Xenopus Proteins / genetics*
  • Xenopus Proteins / metabolism*


  • CDX2 Transcription Factor
  • Cdx4 protein, Xenopus
  • Fetal Proteins
  • Homeodomain Proteins
  • Transcription Factors
  • Xenopus Proteins
  • Xtcad2 protein, Xenopus
  • cdx2 protein, Xenopus