Comparison of two different preparations of HIV immune globulin for efficiency of neutralization of HIV type 1 primary isolates

AIDS Res Hum Retroviruses. 2002 Jan 1;18(1):49-56. doi: 10.1089/088922202753394718.

Abstract

The objective of this study was to compare the virus-neutralizing ability of two different preparations of HIV immune globulin (HIVIG) isolated from human plasma units that were selected according to two different criteria. The first preparation, designated NYBC-HIVIG, was isolated from plasmas with high neutralizing antibody titers against HIV-1. The second preparation, designated NABI-HIVIG, was isolated from plasma with high titers of antibody to the HIV-1 p24 antigen. A panel of primary HIV-1 isolates was phenotypically characterized by their ability to induce syncytia in CEM-SS cells. Neutralization of this panel of primary isolates by the two HIVIG preparations was assessed in HeLa-MAGI-CCR5 cells, utilizing a luminescence-based assay. In addition, the reactivities of these two preparations with a panel of HIV-1 gp120 proteins, V3 loop peptides, and HIV-1 p24 antigen were determined. Both HIVIG preparations were shown to neutralize all virus isolates tested. However, doses of NABI-HIVIG required for 50% virus neutralization were 2.2- to 4.4- fold (mean, 3.2-fold) higher than the required doses of NYBC-HIVIG. Comparative antigen-binding assays showed that, although NABI-HIVIG possessed higher titers of antibody to HIV-1 p24, NYBC-HIVIG generally contained higher titers of antibody to HIV-1 gp120 and V3 peptides. These experiments show that the criteria used for selection of source plasmas for isolation of HIVIG can influence the effective concentration of virus-neutralizing antibody present in the final immunoglobulin preparation, and may determine the doses required for clinical efficacy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Enzyme-Linked Immunosorbent Assay
  • HIV Antibodies / immunology
  • HIV Antibodies / isolation & purification*
  • HIV Core Protein p24 / immunology
  • HIV Envelope Protein gp120 / immunology
  • HIV Infections / blood
  • HIV Infections / immunology*
  • HIV-1 / immunology*
  • Humans
  • Immunoglobulin G / immunology
  • Immunoglobulin G / isolation & purification
  • Neutralization Tests
  • Peptide Fragments / immunology
  • Recombinant Proteins / immunology
  • Viral Proteins / immunology

Substances

  • HIV Antibodies
  • HIV Core Protein p24
  • HIV Envelope Protein gp120
  • HIV envelope protein gp120 (305-321)
  • Immunoglobulin G
  • Peptide Fragments
  • Recombinant Proteins
  • Viral Proteins