The Sm-like Hfq Protein Increases OxyS RNA Interaction With Target mRNAs

Mol Cell. 2002 Jan;9(1):11-22. doi: 10.1016/s1097-2765(01)00437-3.

Abstract

The Escherichia coli host factor I, Hfq, binds to many small regulatory RNAs and is required for OxyS RNA repression of fhlA and rpoS mRNA translation. Here we report that Hfq is a bacterial homolog of the Sm and Sm-like proteins integral to RNA processing and mRNA degradation complexes in eukaryotic cells. Hfq exhibits the hallmark features of Sm and Sm-like proteins: the Sm1 sequence motif, a multisubunit ring structure (in this case a homomeric hexamer), and preferential binding to polyU. We also show that Hfq increases the OxyS RNA interaction with its target messages and propose that the enhancement of RNA-RNA pairing may be a general function of Hfq, Sm, and Sm-like proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Autoantigens / genetics*
  • Autoantigens / metabolism
  • Bacterial Proteins / genetics
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Escherichia coli
  • Escherichia coli Proteins*
  • Host Factor 1 Protein
  • Humans
  • Integration Host Factors
  • Molecular Sequence Data
  • Protein Binding
  • Protein Biosynthesis*
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Ribonucleoproteins, Small Nuclear / genetics
  • Ribonucleoproteins, Small Nuclear / metabolism
  • Sequence Alignment
  • Sigma Factor / genetics
  • Trans-Activators / genetics
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • snRNP Core Proteins

Substances

  • Autoantigens
  • Bacterial Proteins
  • Carrier Proteins
  • Escherichia coli Proteins
  • Host Factor 1 Protein
  • Integration Host Factors
  • RNA, Messenger
  • RNA-Binding Proteins
  • Repressor Proteins
  • Ribonucleoproteins, Small Nuclear
  • Sigma Factor
  • Trans-Activators
  • Transcription Factors
  • integration host factor, E coli
  • oxyS protein, E coli
  • sigma factor KatF protein, Bacteria
  • snRNP Core Proteins
  • fhlA protein, E coli