Relative contributions of bipolar cell and amacrine cell inputs to light responses of ON, OFF and ON-OFF retinal ganglion cells

Vision Res. 2002 Jan;42(1):19-27. doi: 10.1016/s0042-6989(01)00258-9.

Abstract

Light-evoked postsynaptic currents (lePSCs) were recorded from ON, OFF and ON-OFF ganglion cells in dark-adapted salamander retinal slices under voltage clamp conditions, and the cell morphology was examined using Lucifer yellow fluorescence with confocal microscopy. The current-voltage relations of the lePSCs in all three types of ganglion cells are approximately linear within the cells' physiological range. The average chloride/cation conductance ratio (Deltag(Cl)(NR)/Deltag(C)(NR)) of the lePSCs is near 3, suggesting that ganglion cell light responses are associated with a greater postsynaptic conductance change at the amacrine-ganglion cell inhibitory synapses than at the bipolar-ganglion cell excitatory synapses. By comparing the charge transfer of lePSCs in normal Ringer's and in picrotoxin+strychnine+Imidazole-4-acidic acid, we found that the GABAergic and glycinergic amacrine-bipolar cell feedback synapses decreased the light-induced glutamatergic vesicle release from bipolar cells to all ganglion cells, and the degree of release reduction varied widely from ganglion cell to ganglion cell, with a range of 3-28 fold.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Ambystomatidae
  • Animals
  • Chlorides / physiology
  • Electric Conductivity
  • Ganglia, Sensory / physiology*
  • Imidazoles / pharmacology
  • Microscopy, Confocal
  • Patch-Clamp Techniques
  • Picrotoxin / pharmacology
  • Retina / physiology*
  • Strychnine / pharmacology
  • Synaptic Transmission / physiology
  • Vision, Ocular / physiology*

Substances

  • Chlorides
  • Imidazoles
  • Picrotoxin
  • Strychnine