Peptide differential display of serum-free conditioned medium from cancer cell lines

Kae Sato; Kazuki Sasaki; Ming-Sound Tsao; Ken Yamaguchi

doi:10.1016/s0304-3835(01)00755-8

Peptide differential display of serum-free conditioned medium from cancer cell lines

Cancer Lett. 2002 Feb 25;176(2):199-203. doi: 10.1016/s0304-3835(01)00755-8.

Authors

Kae Sato¹, Kazuki Sasaki, Ming-Sound Tsao, Ken Yamaguchi

Affiliation

¹ Growth Factor Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan.

PMID: 11804748
DOI: 10.1016/s0304-3835(01)00755-8

Abstract

Conditioned medium (CM) from cultured cells is a source for screening small peptides of therapeutic or diagnostic value in cancer research. Mass spectrometry has recently enabled the profiling of peptides present in biological samples. We report a single-step extraction method to increase a chance to discover small peptides with a starting volume of 750 microl of serum-free CM. In combination with protein chip mass spectrometry, our protocol will contribute to the discovery of target peptides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Ion Exchange
Culture Media, Serum-Free / pharmacology*
Gene Expression Profiling / methods*
Humans
Hydrogen-Ion Concentration
Mass Spectrometry / methods*
Oligonucleotide Array Sequence Analysis / methods*
Pancreatic Neoplasms / metabolism
Peptides / chemistry*
Tumor Cells, Cultured

Substances

Culture Media, Serum-Free
Peptides