Peptide differential display of serum-free conditioned medium from cancer cell lines

Cancer Lett. 2002 Feb 25;176(2):199-203. doi: 10.1016/s0304-3835(01)00755-8.

Abstract

Conditioned medium (CM) from cultured cells is a source for screening small peptides of therapeutic or diagnostic value in cancer research. Mass spectrometry has recently enabled the profiling of peptides present in biological samples. We report a single-step extraction method to increase a chance to discover small peptides with a starting volume of 750 microl of serum-free CM. In combination with protein chip mass spectrometry, our protocol will contribute to the discovery of target peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Ion Exchange
  • Culture Media, Serum-Free / pharmacology*
  • Gene Expression Profiling / methods*
  • Humans
  • Hydrogen-Ion Concentration
  • Mass Spectrometry / methods*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Pancreatic Neoplasms / metabolism
  • Peptides / chemistry*
  • Tumor Cells, Cultured

Substances

  • Culture Media, Serum-Free
  • Peptides