Analysis of genetic heterogeneity in the HCAR adenovirus-binding Ig1 domain in a Caucasian Flemish population

BMC Genet. 2002;3:1. doi: 10.1186/1471-2156-3-1. Epub 2002 Jan 10.

Abstract

Background: Polymorphisms in the gene that encodes the human cellular receptor for group B coxsackieviruses and adenoviruses (HCAR) could be responsible for differences in susceptibility to infections with these pathogens. Moreover, adenovirus subgroup C-mediated gene therapy could be influenced by mutations in the coding exons for the aminoterminal immunoglobulin-like 1 (Ig1) domain, which is the essential component for adenovirus fiber knob binding.

Results: Using two primersets in the adjacent intron sequences, HCAR exons 2 and 3, which comprise the full-length Ig1 domain, were amplified by polymerase chain reactions in 108 unselected and unrelated healthy Belgian volunteers. After nucleotide sequencing, no polymorphisms could be demonstrated in the adenovirus-binding Ig1 exons 2 and 3 of the HCAR gene.

Conclusions: The adenovirus-binding Ig1 domain seems to be a highly conserved region in the Caucasian population which is a reassuring finding regarding adenovector-based gene therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Belgium
  • Conserved Sequence
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein
  • European Continental Ancestry Group / genetics*
  • Exons
  • Humans
  • Immunoglobulins / chemistry*
  • Introns
  • Molecular Sequence Data
  • Polymorphism, Genetic*
  • Protein Structure, Tertiary
  • Receptors, Virus / chemistry*
  • Receptors, Virus / genetics*

Substances

  • CLMP protein, human
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein
  • Immunoglobulins
  • Receptors, Virus

Associated data

  • GENBANK/AF109644
  • GENBANK/AF109645
  • GENBANK/AF109646
  • GENBANK/AF200465
  • GENBANK/Y07593
  • GENBANK/Y10320