From genome to function: the Arabidopsis aquaporins

Genome Biol. 2002;3(1):RESEARCH0001. doi: 10.1186/gb-2001-3-1-research0001. Epub 2001 Dec 7.


Background: In the post-genomic era newly sequenced genomes can be used to deduce organismal functions from our knowledge of other systems. Here we apply this approach to analyzing the aquaporin gene family in Arabidopsis thaliana. The aquaporins are intrinsic membrane proteins that have been characterized as facilitators of water flux. Originally termed major intrinsic proteins (MIPs), they are now also known as water channels, glycerol facilitators and aqua-glyceroporins, yet recent data suggest that they facilitate the movement of other low-molecular-weight metabolites as well.

Results: The Arabidopsis genome contains 38 sequences with homology to aquaporin in four subfamilies, termed PIP, TIP, NIP and SIP. We have analyzed aquaporin family structure and expression using the A. thaliana genome sequence, and introduce a new NMR approach for the purpose of analyzing water movement in plant roots in vivo.

Conclusions: Our preliminary data indicate a strongly transcellular component for the flux of water in roots.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Aquaporins / chemistry
  • Aquaporins / genetics*
  • Aquaporins / physiology
  • Arabidopsis / genetics*
  • Arabidopsis / physiology
  • Biological Transport
  • Genes, Plant / genetics
  • Genome, Plant*
  • Magnetic Resonance Spectroscopy
  • Models, Biological
  • Molecular Sequence Data
  • Molecular Structure
  • Phylogeny
  • Water / metabolism


  • Aquaporins
  • Water