Proteomic analysis of human prostate cancer

Mol Carcinog. 2002 Jan;33(1):9-15. doi: 10.1002/mc.10019.


Proteomics is a promising approach in the identification of proteins and biochemical pathways involved in tumorigenesis. In an effort to discover such proteins and pathways that are deregulated in prostate tumorigenesis, cellular proteomes of matched normal prostate epithelial cells and high-grade prostate cancer cells were analyzed by tissue microdissection, two-dimensional electrophoresis, and mass spectrometry. Forty protein alterations were detected in the tumors; however, the majority of these changes were not shared among the 12 neoplasms. In contrast, parallel cDNA microarray analysis identified a number of common gene expression changes. The marked heterogeneity of the observed protein alterations may have significance with regard to tumor biology and research strategies for molecular profiling analyses of human prostate cancer.

Publication types

  • Comparative Study

MeSH terms

  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Mass Spectrometry
  • Neoplasm Proteins / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology
  • Proteome
  • Tropomyosin / metabolism
  • Trypsin / metabolism


  • Neoplasm Proteins
  • Proteome
  • Tropomyosin
  • Trypsin