Production of Nuclear Transfer-Derived Swine That Express the Enhanced Green Fluorescent Protein

Anim Biotechnol. 2001 Nov;12(2):173-81. doi: 10.1081/abio-100108344.

Abstract

The ability to add or delete specific genes in swine will likely provide considerable benefits not just to agriculture but also to medicine, where pigs have potential as models for human disease and as organ donors. Here we have transferred nuclei from a genetically modified fibroblast cell line to porcine oocytes, matured in vitro under defined culture conditions, to create piglets expressing enhanced green fluorescent protein. The nuclear transfer-derived piglets were of normal size, although some mild symptoms of "large offspring syndrome" were evident. These experiments represent a next step towards creating swine with more useful genetic modifications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Agriculture
  • Animals
  • Animals, Genetically Modified*
  • Animals, Newborn
  • Body Constitution
  • Cell Nucleus
  • Cloning, Organism / methods*
  • Female
  • Fibroblasts
  • Green Fluorescent Proteins
  • Luminescent Proteins / biosynthesis*
  • Male
  • Oocytes
  • Swine

Substances

  • Luminescent Proteins
  • Green Fluorescent Proteins