Lack of association between the -260 C-->T promoter polymorphism of the endotoxin receptor CD14 gene and the CD14 density of unstimulated human monocytes and soluble CD14 plasma levels

Intensive Care Med. 2001 Nov;27(11):1770-5. doi: 10.1007/s001340101106. Epub 2001 Oct 30.


Objective: CD14 is a receptor for endotoxin and binds components of Gram-positive and Gram-negative bacteria. CD14-bearing monocytes respond to stimulation with the increased synthesis and release of cytokines. The recently described -260 C-->T promoter polymorphism of the CD14 gene has been found to be related to a risk of myocardial infarction. This study evaluated the role of this polymorphism in the expression of monocyte and soluble CD14. Moreover, the effect of the CD14 -260 genotypes for the ex vivo TNF-alpha response to endotoxin was analyzed in whole blood.

Patients and participants: Ninety-five healthy blood donors were studied.

Measurements and results: CD14 -260 genotyping was performed by means of a real-time PCR with fluorescence labeled hybridization probes. CD14 expression on human monocytes (mCD14) was assessed by fluorescence-activated cell sorting analysis with anti-CD14 monoclonal antibodies. Plasma levels of soluble CD14 (sCD14) were measured by ELISA. The TNF-alpha synthesis was determined by chemiluminescence in whole blood after endotoxin stimulation. There were no differences in mCD14 density, sCD14 levels, or the tumor necrosis factor-alpha concentrations between individuals with the three different CD14 -260 genotypes CC, CT, and TT.

Conclusions: The CD14 -260 polymorphism does not affect the CD14 expression of unstimulated circulating monocytes or soluble CD14 plasma levels.

MeSH terms

  • Adult
  • Aged
  • Antibodies, Monoclonal
  • Chi-Square Distribution
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Genotype
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lipopolysaccharide Receptors / blood*
  • Lipopolysaccharide Receptors / genetics*
  • Lipopolysaccharide Receptors / immunology
  • Luminescent Measurements
  • Male
  • Middle Aged
  • Monocytes / immunology*
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Promoter Regions, Genetic
  • Statistics, Nonparametric
  • Tumor Necrosis Factor-alpha / metabolism


  • Antibodies, Monoclonal
  • Lipopolysaccharide Receptors
  • Tumor Necrosis Factor-alpha