A role for HNF-3 in the regulation of the HNF-1 gene of the Atlantic salmon

Mol Genet Genomics. 2002 Jan;266(5):832-7. doi: 10.1007/s00438-001-0602-0. Epub 2001 Nov 7.


Hepatocyte nuclear factors -1 (HNF-1) and -3 (HNF-3) are hepatocyte-enriched transcription factors that are central to the establishment and maintenance of the liver phenotype in vertebrates. In the present study we demonstrate that, in the Atlantic salmon, asHNF-3 regulates the expression of the gene for asHNF-1. Multiple putative binding sites for asHNF-3 were identified within the 5' flanking region of the HNF-1 gene using a computer-based algorithm, and these were confirmed to be functional by electrophoretic mobility shift assays. In transient transfection assays it was shown that co-expression of asHNF-3 leads to a decrease in the promoter activity of the 5' flanking region of the asHNF-1 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • Cell Line
  • Consensus Sequence
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / genetics*
  • Gene Expression Regulation
  • Genes, Reporter
  • Hepatocyte Nuclear Factor 1
  • Hepatocyte Nuclear Factor 1-alpha
  • Hepatocyte Nuclear Factor 1-beta
  • Hepatocyte Nuclear Factor 3-gamma
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Salmo salar / genetics*
  • Transcription Factors / genetics*
  • Transfection


  • DNA, Complementary
  • DNA-Binding Proteins
  • FOXA3 protein, human
  • HNF1A protein, human
  • HNF1B protein, human
  • Hepatocyte Nuclear Factor 1-alpha
  • Nuclear Proteins
  • Transcription Factors
  • Hepatocyte Nuclear Factor 1
  • Hepatocyte Nuclear Factor 3-gamma
  • Hepatocyte Nuclear Factor 1-beta