The murine orthologue of the Golgi-localized TPTE protein provides clues to the evolutionary history of the human TPTE gene family

Hum Genet. 2001 Dec;109(6):569-75. doi: 10.1007/s004390100607. Epub 2001 Oct 27.


The human TPTE gene encodes a testis-specific protein that contains four potential transmembrane domains and a protein tyrosine phosphatase motif, and shows homology to the tumor suppressor PTEN/MMAC1. Chromosomal mapping revealed multiple copies of the TPTE gene present on the acrocentric chromosomes 13, 15, 21 and 22, and the Y chromosome. Zooblot analysis suggests that mice may possess only one copy of TPTE. In the present study, we report the isolation and initial characterization of the full-length cDNA of the mouse homologue Tpte. At least three different mRNA transcripts ( Tpte.a, b, c) are produced via alternative splicing, encoding predicted proteins that would contain four potential transmembrane domains and a protein tyrosine phosphatase motif. Transfection of a 5'EGFP-TPTE fusion protein in Hela cells revealed an intracellular localization within the Golgi apparatus. Tpte was mapped by radiation hybrid to a region of mouse chromosome 8 that shows conserved synteny with human 13q14.2-q21 between NEK3 and SGT1. This region of the human genome was found to contain a partial, highly diverged copy of TPTE that is likely to represent the ancestral copy from which the other copies of TPTE arose through duplication events. The Y chromosome copy of TPTE is a pseudogene and is not therefore involved in the testis expression of this gene family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Cell Compartmentation
  • Chromosome Mapping
  • Chromosomes, Human, Pair 13
  • Evolution, Molecular*
  • Golgi Apparatus / chemistry*
  • Humans
  • Male
  • Membrane Proteins / genetics*
  • Mice
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • PTEN Phosphohydrolase
  • Phosphoric Monoester Hydrolases / genetics
  • Phylogeny
  • Protein Tyrosine Phosphatases*
  • Sequence Homology, Amino Acid
  • Tumor Suppressor Proteins / genetics
  • Vertebrates / classification
  • Vertebrates / genetics


  • Membrane Proteins
  • Tumor Suppressor Proteins
  • Phosphoric Monoester Hydrolases
  • Protein Tyrosine Phosphatases
  • TPTE protein, human
  • PTEN Phosphohydrolase
  • PTEN protein, human