JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes

Nat Immunol. 2002 Feb;3(2):151-8. doi: 10.1038/ni755. Epub 2002 Jan 14.


Inflammatory recruitment of leukocytes is governed by dynamic interactions between integrins and endothelial immunoglobulin superfamily (IgSF) proteins. We have identified the IgSF member junctional adhesion molecule 1 (JAM-1) as a ligand of the beta(2) integrin lymphocyte function-associated antigen 1 (LFA-1). Under static and physiological flow conditions, JAM-1 contributed to LFA-1-dependent transendothelial migration of T cells and neutrophils as well as LFA-1-mediated arrest of T cells. The latter was triggered by chemokines on endothelium that was stimulated with cytokines to redistribute JAM-1 from the tight junctions. Transfectants expressing JAM-1 supported LFA-1-mediated adhesion of leukocytes, which required the membrane-proximal Ig-like domain 2 of JAM-1. Thus, JAM-1 is a counter-receptor for LFA-1 that is ideally situated to guide and control transmigration during leukocyte recruitment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • CD18 Antigens / metabolism*
  • CHO Cells
  • Cell Adhesion
  • Cell Adhesion Molecules*
  • Chemokine CXCL12
  • Chemokines, CXC / pharmacology
  • Chemotaxis, Leukocyte / immunology*
  • Cricetinae
  • Endothelium, Vascular / immunology*
  • Humans
  • Immunologic Memory
  • Jurkat Cells
  • Ligands
  • Lymphocyte Function-Associated Antigen-1 / metabolism*
  • Membrane Proteins / antagonists & inhibitors
  • Membrane Proteins / immunology
  • Membrane Proteins / metabolism*
  • Neutrophils / immunology
  • Protein Binding
  • Receptors, Cell Surface*
  • T-Lymphocytes / immunology
  • Tetradecanoylphorbol Acetate / pharmacology


  • CD18 Antigens
  • CXCL12 protein, human
  • Cell Adhesion Molecules
  • Chemokine CXCL12
  • Chemokines, CXC
  • F11R protein, human
  • Ligands
  • Lymphocyte Function-Associated Antigen-1
  • Membrane Proteins
  • Receptors, Cell Surface
  • Tetradecanoylphorbol Acetate