Abstract
Bacillus subtilis alpha-amylase was cleaved with cyanogen bromide and the amino terminal sequences of the purified products were determined. The molecular weights of the cyanogen bromide fragments were ascertained on an agarose column equilibrated with 6 M guanidine hydrochloride. The molecular wieghts of these fragments were also calculated from their amino acid compositions. The data obtained bzyme monomer as 48,000.
MeSH terms
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Amino Acid Sequence
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Amino Acids / analysis
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Amylases / analysis*
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Bacillus subtilis / enzymology*
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Carboxypeptidases / pharmacology
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Chromatography, Gel
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Cyanogen Bromide / pharmacology
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Electrophoresis
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Molecular Weight
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Peptide Fragments / analysis*
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Peptide Fragments / isolation & purification
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Peptide Fragments / metabolism
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alpha-Amylases / analysis*
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alpha-Amylases / isolation & purification
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alpha-Amylases / metabolism
Substances
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Amino Acids
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Peptide Fragments
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Amylases
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alpha-Amylases
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Carboxypeptidases
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Cyanogen Bromide