Expression and distribution of calcitonin receptor-like receptor in human hairy skin

Peptides. 2002 Jan;23(1):109-16. doi: 10.1016/s0196-9781(01)00586-1.


Calcitonin gene-related peptide and adrenomedullin exert potent effects in skin but their cellular targets are unknown. This study aimed to identify the cellular location of calcitonin receptor-like receptor (CRLR) which is pharmacologically identical to CGRP receptor-1, a putative molecular target of CGRP and adrenomedullin. RT-PCR analysis of human hairy skin revealed the presence of CRLR mRNA and immunohistochemical analysis, employing a previously characterized polyclonal antibody raised to CRLR, provided novel evidence of the cellular distribution of CRLR. Extensive and specific CRLR-immunostaining was detected in arteriolar smooth muscle and venular endothelium and is consistent with CGRP's putative role in neurogenic inflammation. Novel targets for CGRP and/or adrenomedullin were identified, including capillary endothelium, hair follicles and sweat glands.

MeSH terms

  • Adrenomedullin
  • Arteries / metabolism
  • Calcitonin Gene-Related Peptide / metabolism
  • Calcitonin Receptor-Like Protein
  • Capillaries / metabolism
  • Endothelium, Vascular / metabolism
  • Epithelial Cells / metabolism
  • Fluorescent Antibody Technique, Indirect
  • Hair / metabolism*
  • Hair Follicle / metabolism
  • Humans
  • Immunohistochemistry
  • Muscle, Smooth / metabolism
  • Peptides / metabolism
  • RNA, Messenger / metabolism
  • Receptors, Calcitonin / biosynthesis*
  • Receptors, Calcitonin / chemistry*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin / metabolism*
  • Sweat Glands / metabolism


  • CALCRL protein, human
  • Calcitonin Receptor-Like Protein
  • Peptides
  • RNA, Messenger
  • Receptors, Calcitonin
  • Adrenomedullin
  • Calcitonin Gene-Related Peptide