Investigation of substance P transport across the blood-brain barrier

Peptides. 2002 Jan;23(1):157-65. doi: 10.1016/s0196-9781(01)00592-7.

Abstract

The transport of substance P (SP) was investigated using the bovine brain microvessel endothelial cell culture model of the blood-brain barrier (BBB). The samples were derivatized precolumn with naphthalene dialdehyde, then analyzed by cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detection. SP crossed the BBB in both the apical-to-basolateral and basolateral-to-apical directions through an active transport mechanism. The transport of SP from the apical side was demonstrated to be via transcytosis. The N-terminal (SP(1-4)) and C-terminal (SP(3-11)) fragments were also found to permeate the BBB from the apical side.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biological Transport
  • Biological Transport, Active
  • Blood-Brain Barrier*
  • Cattle
  • Cell Division
  • Cells, Cultured
  • Cyclodextrins / metabolism
  • Electrophoresis, Capillary
  • Endocytosis
  • Endothelium, Vascular / cytology
  • Hydrogen-Ion Concentration
  • Indicators and Reagents / pharmacology
  • Microscopy, Fluorescence
  • Models, Chemical
  • Molecular Sequence Data
  • Naphthalenes / pharmacology
  • Protein Structure, Tertiary
  • Substance P / metabolism*
  • Temperature
  • Time Factors

Substances

  • Cyclodextrins
  • Indicators and Reagents
  • Naphthalenes
  • Substance P
  • 2,3-naphthalenedicarboxaldehyde