Abstract
The transport of substance P (SP) was investigated using the bovine brain microvessel endothelial cell culture model of the blood-brain barrier (BBB). The samples were derivatized precolumn with naphthalene dialdehyde, then analyzed by cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detection. SP crossed the BBB in both the apical-to-basolateral and basolateral-to-apical directions through an active transport mechanism. The transport of SP from the apical side was demonstrated to be via transcytosis. The N-terminal (SP(1-4)) and C-terminal (SP(3-11)) fragments were also found to permeate the BBB from the apical side.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Biological Transport
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Biological Transport, Active
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Blood-Brain Barrier*
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Cattle
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Cell Division
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Cells, Cultured
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Cyclodextrins / metabolism
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Electrophoresis, Capillary
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Endocytosis
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Endothelium, Vascular / cytology
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Hydrogen-Ion Concentration
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Indicators and Reagents / pharmacology
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Microscopy, Fluorescence
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Models, Chemical
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Molecular Sequence Data
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Naphthalenes / pharmacology
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Protein Structure, Tertiary
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Substance P / metabolism*
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Temperature
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Time Factors
Substances
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Cyclodextrins
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Indicators and Reagents
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Naphthalenes
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Substance P
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2,3-naphthalenedicarboxaldehyde