Identification of a dopamine receptor from Caenorhabditis elegans

Neurosci Lett. 2002 Feb 8;319(1):13-6. doi: 10.1016/s0304-3940(01)02477-6.


The neurotransmitter dopamine regulates locomotion and egg laying in the nematode Caenorhabditis elegans. We have cloned a cDNA encoding the C. elegans G protein-coupled receptor (CeDOP1). The deduced amino acid sequence of the cloned cDNA shows high sequence similarities with D1-like dopamine receptors from other species. Three splice variants that differ in the length of the predicted third intracellular loop and C-terminal tail were identified. COS-7 cells transiently transfected with CeDOP1 showed high affinity binding to [(125)I]iodo-lysergic acid diethylamide (K(D)=3.43 +/- 0.83 nM). Dopamine showed the highest affinity (K(i)=0.186 microM) for this receptor among several vertebrate and invertebrate amine neurotransmitters tested, suggesting that the natural ligand for this receptor is dopamine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / physiology
  • Binding, Competitive / drug effects
  • Binding, Competitive / physiology
  • COS Cells
  • Caenorhabditis elegans / metabolism*
  • Cloning, Molecular
  • DNA, Complementary
  • Dopamine / metabolism*
  • Feeding Behavior / physiology
  • Gene Expression Regulation / genetics
  • Iodine Radioisotopes / pharmacokinetics
  • Ligands
  • Lysergic Acid Diethylamide / pharmacokinetics
  • Molecular Sequence Data
  • Nervous System / metabolism*
  • Neurons / metabolism*
  • Receptors, Dopamine D1 / genetics*
  • Receptors, Dopamine D1 / metabolism*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Serotonin Antagonists / pharmacokinetics
  • Transfection


  • DNA, Complementary
  • Iodine Radioisotopes
  • Ligands
  • Receptors, Dopamine D1
  • Serotonin Antagonists
  • Lysergic Acid Diethylamide
  • Dopamine