Abstract
The neurotransmitter dopamine regulates locomotion and egg laying in the nematode Caenorhabditis elegans. We have cloned a cDNA encoding the C. elegans G protein-coupled receptor (CeDOP1). The deduced amino acid sequence of the cloned cDNA shows high sequence similarities with D1-like dopamine receptors from other species. Three splice variants that differ in the length of the predicted third intracellular loop and C-terminal tail were identified. COS-7 cells transiently transfected with CeDOP1 showed high affinity binding to [(125)I]iodo-lysergic acid diethylamide (K(D)=3.43 +/- 0.83 nM). Dopamine showed the highest affinity (K(i)=0.186 microM) for this receptor among several vertebrate and invertebrate amine neurotransmitters tested, suggesting that the natural ligand for this receptor is dopamine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Behavior, Animal / physiology
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Binding, Competitive / drug effects
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Binding, Competitive / physiology
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COS Cells
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Caenorhabditis elegans / metabolism*
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Cloning, Molecular
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DNA, Complementary
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Dopamine / metabolism*
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Feeding Behavior / physiology
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Gene Expression Regulation / genetics
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Iodine Radioisotopes / pharmacokinetics
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Ligands
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Lysergic Acid Diethylamide / pharmacokinetics
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Molecular Sequence Data
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Nervous System / metabolism*
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Neurons / metabolism*
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Receptors, Dopamine D1 / genetics*
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Receptors, Dopamine D1 / metabolism*
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Serotonin Antagonists / pharmacokinetics
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Transfection
Substances
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DNA, Complementary
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Iodine Radioisotopes
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Ligands
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Receptors, Dopamine D1
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Serotonin Antagonists
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Lysergic Acid Diethylamide
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Dopamine