Nucleotide sequence and organization of plasmid pMVSCS1 from Mannheimia varigena: identification of a multiresistance gene cluster

J Antimicrob Chemother. 2002 Feb;49(2):383-6. doi: 10.1093/jac/49.2.383.


Objectives: A small resistance plasmid of Mannheimia varigena was analysed with regard to its gene organization and the development of a multiresistance gene cluster.

Materials and methods: The 5621 bp plasmid pMVSCS1 was transformed into Escherichia coli JM107, cloned and sequenced completely.

Results: Three intact resistance genes, sulII, catAIII and strA, a truncated strB gene and a novel replication gene were identified. A potential recombination site for the integration of catAIII in the spacer region between sulII and strA was identified.

Conclusion: The physical linkage of the plasmid-borne resistance genes organized in a cluster would facilitate the spread of the different resistance genes by co-selection, even in the absence of a direct selective pressure.

MeSH terms

  • Animals
  • Base Sequence / genetics*
  • Bronchopneumonia / microbiology
  • Chloramphenicol / pharmacology
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Gene Order
  • Genes, Bacterial*
  • Multigene Family / genetics*
  • Pasteurellaceae / genetics*
  • Pasteurellaceae / isolation & purification
  • Plasmids / chemistry
  • Plasmids / genetics*
  • Streptomycin / pharmacology
  • Sulfonamides / pharmacology
  • Swine


  • Sulfonamides
  • Chloramphenicol
  • Streptomycin