Identification of type II collagen peptide 261-273-specific T cell clones in a patient with relapsing polychondritis

Arthritis Rheum. 2002 Jan;46(1):238-44. doi: 10.1002/1529-0131(200201)46:1<238::AID-ART10030>3.0.CO;2-M.


Objective: To characterize and clone T cells specific for type II collagen (CII) in a patient with relapsing polychondritis (RP) and to establish whether the immunodominant epitope of CII determined in HLA transgenic mice is used in the human autoimmune response to CII.

Methods: T cell responses to CII were examined in a patient with RP, who was heterozygous for the HLA-DR allele DRB1*0101/DRB1*0401. T cell clones were established from this patient and characterized for peptide specificity, class II restriction, cytokine production, and staining with HLA-DRB1*0401 class II tetramers.

Results: A response to CII and the peptide 255-273 was present in this patient. T cells specific for the CII epitope 261-273 were cloned. Evaluation of these clones demonstrated a response to CII 261-273 in the context of both DR alleles. HLA-DR4 CII tetramer did not demonstrate staining of either CII-specific DRB1*0401-restricted T cell clones or a polyclonal population of CII-reactive T cells from this individual.

Conclusion: T cells directed against CII were present in this patient with RP. Also, T cell clones isolated from this individual were found to be specific for the CII peptide 261-273 and were restricted to either the DRB1*0101 or the DRB1*0401 allele. These findings establish that a T cell response directed against CII is present in this patient with RP and that the CII peptide 261-273 plays a role in the human immune response to CII.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Clone Cells / immunology
  • Collagen Type II / analysis*
  • Collagen Type II / immunology*
  • Epitopes
  • Flow Cytometry
  • HLA-DR Antigens / immunology
  • HLA-DRB1 Chains
  • Histocompatibility Testing
  • Humans
  • Male
  • Peptide Fragments / immunology
  • Polychondritis, Relapsing / immunology*
  • Staining and Labeling
  • T-Lymphocytes / chemistry*
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / immunology


  • Collagen Type II
  • Epitopes
  • HLA-DR Antigens
  • HLA-DRB1 Chains
  • HLA-DRB1*04:01 antigen
  • Peptide Fragments