The effects of dietary inorganic chromium on some biochemical parameters were determined in lambs fed either a control diet or a 200-ppb or 400-ppb chromium-supplemented diet. The live weight of the animals were measured and jugular blood samples were collected prior to supplementation (d 0) and on d 20, 40, and 55. On d 55, three animals from each group were slaughtered to measure subcutaneous fat. Sera were analyzed for glucose, triglyceride, total cholesterol, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, total protein, albumin, ALT, AST, and GGT levels. Chromium supplementation had no significant effect on live weight, but subcutaneous fat was reduced significantly in both chromium groups. There was a slight decrease in glucose concentrations in the 200-ppb chromium group, although only the differences on d 55 were significant. Triglyceride levels in both chromium groups were lower than the control group with marked differences in the 400-ppb chromium group. HDL cholesterol levels increased in both treatment groups compare to control, although the differences in the 400-ppb chromium group on d 40 were significant. Serum Cr concentrations slightly but not significantly increased in both chromium groups. No significant differences were found in total and LDL cholesterol, total protein, albumin, ALT, AST, and GGT levels. In conclusion, chromium supplementation may affect carbohydrate and lipid metabolisms and lipid deposition in lambs.