Differential Expression of a Novel Colorectal Cancer Differentiation-Related Gene in Colorectal Cancer

World J Gastroenterol. 2001 Aug;7(4):551-4. doi: 10.3748/wjg.v7.i4.551.

Abstract

Aim: To investigate SBA2 expression in CRC cell lines and surgical specimens of CRC and autologous healthy mucosa.

Methods: Reverse transcription-polymerase chain reaction (RT-PCR) was used for relative quantification of SBA2 mRNA levels in 4 human CRC cell lines with different grades of differentiation and 30 clinical samples. Normalization of the results was achieved by simultaneous amplification of beta-actin as an internal control.

Results: In the exponential range of amplification, fairly good linearity demonstrated identical amplification efficiency for SBA2 and beta-actin (82%). Markedly lower levels of SBA2 mRNA were detectable in tumors, as compared with the coupled normal counterparts P<0.01). SBA2 expression was significantly (0.01>P < 0.05) correlated with the grade of differentiation in CRC, with relatively higher levels in well-differentiated samples and lower in poorly-differentiated cases. Of the 9 cases with lymph nodes affected, 78% (7/9) had reduced SBA2 mRNA expression in contrast to 24% (5/21) in non-metastasis samples 0.01>P<0.05).

Conclusion: SBA2 gene might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological features need further studies.

MeSH terms

  • Biomarkers, Tumor
  • Cell Differentiation / genetics
  • Colorectal Neoplasms / genetics
  • Colorectal Neoplasms / pathology*
  • Colorectal Neoplasms / physiopathology*
  • DNA, Complementary
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Polymerase Chain Reaction
  • Tumor Cells, Cultured

Substances

  • Biomarkers, Tumor
  • DNA, Complementary
  • Neoplasm Proteins

Associated data

  • GENBANK/AF229181