Conserved guanine-guanine stacking in tetraplex and duplex DNA

Eur Biophys J. 2001 Dec;30(7):555-8. doi: 10.1007/s002490100174.

Abstract

Using a series of suitably chosen oligonucleotides, we demonstrate that the DNA duplex of d(CCCCGGGG) provides an almost identical CD spectrum as the parallel-stranded tetraplex of d(GGGG). The CD spectra are very sensitive to base stacking in DNA so that the above observation indicates that guanine-guanine stacking is essentially the same within the duplex of d(CCCCGGGG) and the tetraplex of d(GGGG). A very similar CD spectrum is also provided by the A-form of d(CCCCGGGG) induced by trifluoroethanol. These results reveal that guanine-guanine stacking is a structural invariant conserved in various nucleic acid conformers. The structural invariance is likely to cohere with evolution of the genetic molecules and be important for fundamental functions, e.g. initiation of transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Circular Dichroism
  • DNA / chemistry*
  • Electrophoresis, Polyacrylamide Gel
  • Guanine / chemistry*
  • Nucleic Acid Conformation
  • Nucleic Acids / chemistry
  • Polytetrafluoroethylene / pharmacology
  • Protein Conformation
  • Transcription, Genetic

Substances

  • Nucleic Acids
  • Guanine
  • Polytetrafluoroethylene
  • DNA