Abstract
We showed in human melanoma cells tryptophan hydroxylase (TPH) and hydroxyindole methyltransferase genes expression with the sequential enzymatic activities of TPH, serotonin (Ser) N-acetyltransferase and hydroxyindole methyltransferase. The presence of the products Ser, 5OH-tryptophan, N-acetylserotonin, melatonin (Mel), 5-methoxytryptamine and 5-methoxytryptophol was documented by liquid chromatography-mass spectrometry. Thus, human melanoma cells can synthesize and metabolize Ser and Mel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylserotonin O-Methyltransferase / genetics
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Acetylserotonin O-Methyltransferase / metabolism
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Chromatography, High Pressure Liquid
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Humans
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Melanoma / enzymology
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Melanoma / genetics
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Melanoma / metabolism*
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Melatonin / metabolism*
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Serotonin / metabolism*
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Spectrometry, Mass, Electrospray Ionization
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Tryptophan / metabolism*
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Tryptophan Hydroxylase / genetics
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Tryptophan Hydroxylase / metabolism
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Tumor Cells, Cultured
Substances
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RNA, Messenger
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Serotonin
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Tryptophan
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Tryptophan Hydroxylase
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Acetylserotonin O-Methyltransferase
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Melatonin