The effect of allergen-induced airway inflammation on airway remodeling in a murine model of allergic asthma

Inflamm Res. 2001 Dec;50(12):616-24. doi: 10.1007/PL00000243.


Objective and design: We examined the effect of airway inflammation on airway remodeling and bronchial responsiveness in a mouse model of allergic asthma.

Materials and methods: BALB/c mice were sensitized to ovalbumin (OA), and exposed to aerosolized OA (0.01, 0.1 and 1%). Twenty-four hours after the final antigen challenge, bronchial responsiveness was measured, and bronchoalveolar lavage (BAL) and histological examinations were carried out.

Results: Repeated antigen exposure induced airway inflammation, IgE/IgG1 responses, epithelial changes, collagen deposition in the lungs, subepithelial fibrosis associated with increases in the amount of transforming growth factor (TGF)-beta1 in BAL fluid (BALF), and bronchial hyperresponsiveness to acetylcholine. The number of eosinophils in BALF was significantly correlated with TGF-beta1 production in BALF and the amount of hydroxyproline. Furthermore, significant correlations were found between these fibrogenic parameters and the bronchial responsiveness.

Conclusion: These findings demonstrated that in this murine model airway eosinophilic inflammation is responsible for the development of airway remodeling as well as bronchial hyperresponsiveness in allergic bronchial asthma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / pharmacology
  • Allergens / toxicity*
  • Animals
  • Asthma / etiology
  • Asthma / pathology*
  • Bronchial Hyperreactivity / pathology
  • Bronchoalveolar Lavage Fluid / cytology
  • Collagen / pharmacokinetics
  • Cytokines / biosynthesis
  • Epithelial Cells / pathology
  • Female
  • Hydroxyproline / metabolism
  • Hypersensitivity / complications
  • Hypersensitivity / pathology*
  • Immunoglobulin E / biosynthesis
  • Immunoglobulin G / biosynthesis
  • Immunoglobulins / biosynthesis
  • Immunoglobulins / genetics
  • Lung / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Respiratory System / pathology*
  • Transforming Growth Factor beta / metabolism


  • Allergens
  • Cytokines
  • Immunoglobulin G
  • Immunoglobulins
  • Transforming Growth Factor beta
  • Immunoglobulin E
  • Collagen
  • Acetylcholine
  • Hydroxyproline