Simple procedure for rapid identification of Vibrio cholerae from the aquatic environment

Appl Environ Microbiol. 2002 Feb;68(2):995-8. doi: 10.1128/AEM.68.2.995-998.2002.

Abstract

Biochemical tests commonly used to screen for Vibrio cholerae in environmental samples were evaluated, and we found that a combination of alkaline peptone enrichment followed by streaking on thiosulfate citrate bile salts sucrose agar and testing for arginine dihydrolase activity and esculin hydrolysis was an effective rapid technique to screen for aquatic environmental V. cholerae. This technique provided 100% sensitivity and > or =70% specificity.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Culture Media
  • Peptones
  • Plankton / microbiology
  • Seawater / microbiology*
  • Sensitivity and Specificity
  • Time Factors
  • Vibrio cholerae / classification*
  • Vibrio cholerae / genetics
  • Vibrio cholerae / isolation & purification*
  • Vibrio cholerae / metabolism

Substances

  • Culture Media
  • Peptones