Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A

Kenji Hirose; Ken-Ichiro Itoh; Hiroshi Nakajima; Takayuki Kurazono; Masanori Yamaguchi; Kazuo Moriya; Takayuki Ezaki; Yoshiaki Kawamura; Kazumichi Tamura; Haruo Watanabe

doi:10.1128/JCM.40.02.633-636.2002

Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A

J Clin Microbiol. 2002 Feb;40(2):633-6. doi: 10.1128/JCM.40.02.633-636.2002.

Authors

Kenji Hirose¹, Ken-Ichiro Itoh, Hiroshi Nakajima, Takayuki Kurazono, Masanori Yamaguchi, Kazuo Moriya, Takayuki Ezaki, Yoshiaki Kawamura, Kazumichi Tamura, Haruo Watanabe

Affiliation

¹ Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. hirosek@nih.go.jp

Abstract

The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS), fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Bacterial / genetics*
Bacterial Proteins / genetics
Carbohydrate Epimerases / genetics
Flagellin / genetics
Humans
Paratyphoid Fever / microbiology
Polymerase Chain Reaction / methods*
Salmonella paratyphi A / classification*
Salmonella paratyphi A / genetics
Salmonella paratyphi A / immunology
Salmonella typhi / classification*
Salmonella typhi / genetics
Salmonella typhi / immunology
Transaminases / genetics
Transcription Factors / genetics
Typhoid Fever / microbiology

Substances

Antigens, Bacterial
Bacterial Proteins
Transcription Factors
tviA protein, Salmonella typhi
Flagellin
Transaminases
Carbohydrate Epimerases
paratose synthase
perosamine synthetase