WAC, a novel WW domain-containing adapter with a coiled-coil region, is colocalized with splicing factor SC35

Genomics. 2002 Jan;79(1):87-94. doi: 10.1006/geno.2001.6684.

Abstract

WW domains mediate protein-protein interactions in many intracellular processes. In pre-mRNA splicing, WW domains participate in cross-intron bridging. These WW domains are characterized by a central aromatic block of three tyrosine residues. We identified a novel protein containing the same type of WW domain. The gene encoding the protein, named WAC, is located in human chromosome 10p11.2-10p12.1. A Drosophila melanogaster WAC homolog (CG8949) was identified as a Rosetta stone protein. Domain fusion analysis of the Rosetta stone protein linked WAC to splicing factor SNRP70. WAC existed mainly in a tyrosine-phosphorylated form. Immunofluorescence analysis colocalized WAC with SC35, the marker for pre-mRNA splicing machinery. Our analysis suggests that WAC represents a novel member of WW-domain-containing proteins for RNA processing.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Nucleus / genetics
  • Chromosome Mapping
  • Chromosomes, Human, Pair 10*
  • Cloning, Molecular
  • Drosophila melanogaster / genetics
  • Humans
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Polycystic Kidney Diseases / genetics
  • Protein Structure, Secondary / genetics
  • Protein Structure, Tertiary / genetics
  • Proteins / genetics
  • RNA Processing, Post-Transcriptional / genetics
  • Ribonucleoproteins*
  • Sequence Analysis, Protein
  • Serine-Arginine Splicing Factors
  • TRPP Cation Channels

Substances

  • Nuclear Proteins
  • Proteins
  • Ribonucleoproteins
  • SRSF2 protein, mouse
  • TRPP Cation Channels
  • polycystic kidney disease 1 protein
  • SRSF2 protein, human
  • Serine-Arginine Splicing Factors

Associated data

  • GENBANK/AF320996
  • GENBANK/AI582775