Experimentally determined sequence requirement of ACGT-containing abscisic acid response element

Plant Cell Physiol. 2002 Jan;43(1):136-40. doi: 10.1093/pcp/pcf014.

Abstract

The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGT-core of motif A, the principal ABRE of the rice gene, OSEM: This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology*
  • Base Sequence
  • Binding Sites / genetics
  • Cloning, Molecular
  • DNA, Plant / genetics
  • DNA-Binding Proteins / genetics
  • G-Box Binding Factors
  • Gene Expression Regulation / drug effects
  • Glucuronidase / drug effects
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Mutation
  • Oryza / drug effects
  • Oryza / genetics
  • Plant Growth Regulators / pharmacology*
  • Plant Proteins / genetics
  • Promoter Regions, Genetic / genetics
  • Recombinant Fusion Proteins / drug effects
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Response Elements / genetics*
  • Transcription Factors / genetics

Substances

  • DNA, Plant
  • DNA-Binding Proteins
  • G-Box Binding Factors
  • Osem protein, Oryza sativa
  • Plant Growth Regulators
  • Plant Proteins
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Abscisic Acid
  • Glucuronidase