Protective effects of puerariaeflos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC

Jpn J Pharmacol. 2001 Dec;87(4):338-42. doi: 10.1254/jjp.87.338.


Puerariaeflos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Deterrents / pharmacology*
  • Apoptosis / drug effects*
  • Brain Neoplasms / pathology*
  • DNA Fragmentation / drug effects
  • Ethanol / antagonists & inhibitors*
  • Flow Cytometry
  • Humans
  • Neuroblastoma / pathology*
  • Plant Extracts / pharmacology
  • Plants, Medicinal / chemistry*
  • Pueraria / chemistry*
  • Tumor Cells, Cultured


  • Alcohol Deterrents
  • Plant Extracts
  • Ethanol