Mandibular reconstruction with prefabricated vascularized bone grafts using recombinant human osteogenic protein-1: an experimental study in miniature pigs. Part II: transplantation

Int J Oral Maxillofac Surg. 2001 Dec;30(6):469-78. doi: 10.1054/ijom.2000.0008.


Osteogenic Protein-1 (rhOP-1), also called bone morphogenetic protein-7 (BMP-7), is osteoinductive. The aim of this study was to present a new surgical technique: the prefabrication of a vascularized bone graft using rhOP-1 and its microsurgical transplantation. During 6 weeks, osteomuscular grafts were prefabricated in the latissimus dorsi muscle of five adult minipigs. Six hundred micrograms rhOP-1 on a carrier of xenogenic bone mineral in block form were used. The grafts were transplanted into defects of the mandibular angles performing a microsurgical anastomosis and using miniplates for fixation. Identical defects of the contralateral side were treated by direct application of 600 microg rhOP-1 and xenogenic bone mineral. A polychrome sequential labelling was applied. After transplantation the bone stayed viable, demonstrated by continuous apposition of fluorochromes (non-decalcified histologic sections) and bone scintigraphy. The reconstructive result was significantly superior in the prefabrication technique, assessed by histology and computerized tomography (CT). In conclusion, the method has a potential to become a clinical alternative for conventional vascularized bone grafts.

MeSH terms

  • Anastomosis, Surgical
  • Animals
  • Bone Matrix / transplantation
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins / therapeutic use*
  • Bone Plates
  • Bone Screws
  • Bone Substitutes / therapeutic use
  • Bone Transplantation / methods*
  • Bone Transplantation / pathology
  • Female
  • Fluorescent Dyes
  • Graft Survival
  • Humans
  • Image Processing, Computer-Assisted
  • Mandible / pathology
  • Mandible / surgery*
  • Microradiography
  • Microscopy, Fluorescence
  • Microsurgery
  • Minerals / therapeutic use
  • Muscle, Skeletal / transplantation
  • Radiopharmaceuticals
  • Recombinant Proteins
  • Surgical Flaps
  • Swine
  • Swine, Miniature
  • Technetium Tc 99m Medronate / analogs & derivatives*
  • Tomography, Emission-Computed, Single-Photon
  • Tomography, X-Ray Computed
  • Transforming Growth Factor beta / therapeutic use*
  • Transplantation, Heterologous


  • BMP7 protein, human
  • Bio-Oss
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins
  • Bone Substitutes
  • Fluorescent Dyes
  • Minerals
  • Radiopharmaceuticals
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • technetium Tc 99m hydroxymethylene diphosphonate
  • Technetium Tc 99m Medronate