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. 2002 Feb;59(3):347-60.
doi: 10.1016/s0031-9422(01)00432-0.

Metabolic Profiling of Saponins in Medicago Sativa and Medicago Truncatula Using HPLC Coupled to an Electrospray Ion-Trap Mass Spectrometer

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Metabolic Profiling of Saponins in Medicago Sativa and Medicago Truncatula Using HPLC Coupled to an Electrospray Ion-Trap Mass Spectrometer

David V Huhman et al. Phytochemistry. .

Abstract

Triterpene saponins isolated from Medicago sativa (alfalfa) and Medicago truncatula roots were separated, profiled and identified using an optimized, reversed-phase HPLC with on-line photodiode array detection and electrospray ionization mass spectrometry method (HPLC/PDA/ESI/MS). ESI source polarity and solvent conditions were compared. The effects of these parameters on mass spectral attributes were determined. Ion structures were confirmed using tandem mass spectrometry (MS/MS). Fifteen saponins were identified in alfalfa (cultivars Apollo, Radius, and Kleszczewska) based upon negative-ion HPLC/PDA/ESI/MS, HPLC/PDA/ESI/MS/MS and literature data. In addition, the identification of two new malonated saponins in alfalfa are proposed. Negative-ion HPLC/PDA/ESI/MS and HPLC/PDA/ESI/MS/MS spectra were utilized along with HPLC retention times to profile and identify 27 saponins in M. truncatula (cultivar Jemalong, A17). M. truncatula yielded a much more complex mixture of saponins than observed for alfalfa. The authors are not aware of any previous reports identifying saponin glycosides in M. truncatula.

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