Intrinsic differences between the superficial and deep layers of the Xenopus ectoderm control primary neuronal differentiation

Dev Cell. 2002 Feb;2(2):171-82. doi: 10.1016/s1534-5807(02)00113-2.

Abstract

In Xenopus, primary neurons differentiate early, in the deep layer of the neuroectoderm. In contrast, the neural precursors of the superficial layer continue to proliferate. We report that superficial layer precursors differ from deep layer precursors in that they are refractory to the neuronal-promoting activity of bHLH genes, dominant-negative X-Delta-1, FGF-8, or signals from the organizer. In this system, neuronal differentiation is guided by an early established, intrinsic, cell-autonomous difference in the competence of the precursor cells to differentiate. This difference may be controlled in part by ESR6e, a bHLH gene of the Enhancer-of-split family, which is expressed in the superficial layer of the late blastula and when expressed ectopically suppresses primary neurogenesis in the deep layer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Biomarkers / analysis
  • Blastocyst / cytology
  • Blastocyst / metabolism
  • Cell Lineage
  • Cell Polarity
  • Cells, Cultured
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Ectoderm / cytology*
  • Ectoderm / metabolism*
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / embryology*
  • Embryo, Nonmammalian / metabolism
  • Embryonic Induction*
  • Fibroblast Growth Factor 8
  • Fibroblast Growth Factors / genetics
  • Fibroblast Growth Factors / metabolism
  • Gastrula / cytology
  • Gastrula / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Mesoderm / cytology
  • Mesoderm / metabolism
  • Morphogenesis
  • Nervous System / cytology
  • Nervous System / embryology
  • Nervous System / metabolism
  • Neurons / cytology*
  • Neurons / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Repressor Proteins / metabolism
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Xenopus / embryology*
  • Xenopus / metabolism
  • Xenopus Proteins / genetics
  • Xenopus Proteins / metabolism

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Biomarkers
  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • RNA, Messenger
  • Repressor Proteins
  • Transcription Factors
  • Xenopus Proteins
  • delta protein
  • Fibroblast Growth Factor 8
  • Fibroblast Growth Factors