A variation in 3' UTR of hPTP1B increases specific gene expression and associates with insulin resistance

Am J Hum Genet. 2002 Mar;70(3):806-12. doi: 10.1086/339270. Epub 2002 Feb 6.


Protein tyrosine phosphatase 1B (PTP1B) inhibits insulin signaling and, when overexpressed, plays a role in insulin resistance (Ahmad et al. 1997). We identified, in the 3' untranslated region of the PTP1B gene, a 1484insG variation that, in two different populations, is associated with several features of insulin resistance: among male individuals, higher values of the insulin resistance HOMA(IR) index (P=.006), serum triglycerides (P=.0002), and total/HDL cholesterol ratio (P=.025) and, among female individuals, higher blood pressure (P=.01). Similar data were also obtained in a family-based association study by use of sib pairs discordant for genotype (Gu et al. 2000). Subjects carrying the 1484insG variant showed also PTP1B mRNA overexpression in skeletal muscle (6,166 plus minus 1,879 copies/40 ng RNA vs. 2,983 plus minus 1,620; P<.01). Finally, PTP1B mRNA stability was significantly higher (P<.01) in human embryo kidney 293 cells transfected with 1484insG PTP1B, as compared with those transfected with wild-type PTP1B. Our data indicate that the 1484insG allele causes PTP1B overexpression and plays a role in insulin resistance. Therefore, individuals carrying the 1484insG variant might particularly benefit from PTP1B inhibitors, a promising new tool for treatment of insulin resistance (Kennedy and Ramachandran 2000).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics*
  • Adult
  • Blood Glucose / analysis
  • Blood Pressure / genetics
  • Cell Line
  • Cholesterol / blood
  • Dactinomycin / pharmacology
  • Exons / genetics
  • Fasting / blood
  • Female
  • Gene Expression Regulation* / drug effects
  • Gene Frequency
  • Genotype
  • Humans
  • Insulin / blood
  • Insulin Resistance / genetics*
  • Introns / genetics
  • Male
  • Mutation / genetics*
  • Polymorphism, Single Nucleotide / genetics*
  • Protein Tyrosine Phosphatase, Non-Receptor Type 1
  • Protein Tyrosine Phosphatases / genetics*
  • RNA Stability / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Triglycerides / blood


  • 3' Untranslated Regions
  • Blood Glucose
  • Insulin
  • RNA, Messenger
  • Triglycerides
  • Dactinomycin
  • Cholesterol
  • PTPN1 protein, human
  • Protein Tyrosine Phosphatase, Non-Receptor Type 1
  • Protein Tyrosine Phosphatases